September 13, 2023 – A research team led by Shir Filo and Prof. Aviv Mezer of Hebrew University of Jerusalem (HU), and Dr. Tal Shahar, Director of the Neurosurgical Oncology Unit at the Neurosurgery Department at Tel Aviv Sourasky Medical Center unveiled a groundbreaking magnetic resonance imaging (MRI) technology that promises to revolutionize our understanding of iron homeostasis in the human brain. Their research demonstrates the ability to non-invasively assess different molecular iron environments within the brain, shedding light on its vital role in normal brain function, aging, neurodegenerative diseases, and cancer.

Traditional MRI scans provide qualitative images that necessitate subjective interpretation by medical professionals. In contrast, quantitative MRI scans seek to replicate the precision of assessing body temperature accurately when ill, moving beyond qualitative observations like “too hot” or “too cold.” Achieving this involves intricate physical models that amalgamate multiple MRI scans to extract a diverse array of measurements. These MRI measurements are then leveraged to uncover valuable biological insights, akin to a blood test’s ability to reveal our blood’s composition, encompassing proteins, fats, and potential irregularities. One critical aspect of brain function is maintaining the balance, or homeostasis, of iron, crucial for overall health. Imbalances in brain iron levels have implications for various conditions, including neurodegenerative diseases and cancer. Until recently, the non-invasive assessment of the molecular iron environment within the living human brain posed a significant challenge.

MRI 2. Credit Shir Filo Team
MRI 2. Credit Shir Filo Team

The research team from Hebrew University, Tel Aviv Sourasky Medical Center, and Shaare Zedek Medical Center introduces a novel MRI approach based on the quantitative MRI, which can sensitively detect changes in brain iron homeostasis. In their in vitro experiments, this MRI technology revealed distinct paramagnetic properties of key iron compounds, including ferritin, transferrin, and ferrous iron. The real breakthrough came with in vivo MRI scans of patients with brain tumors, operated at the Neurosurgery Department at Shaare Zedek Medical Center where the technology was also validated against ex vivo iron compound quantification of the resected tumors. These ex-vivo transcriptomics and proteomics analyses were done in the lab of Dr. Naomi Habib at the Hebrew University and the lab of Molecular Neuro-oncology at Shaare Zedek Medical Center, directed at the time by Dr. Tal Shahar.

This innovative MRI approach demonstrates sensitivity to changes in iron mobilization capacity across different brain regions and during brain aging. It offers insights into changes in iron homeostasis and iron-related gene expression in pathological tissues. Most notably, it can differentiate between tumor tissue and non-pathological tissue without the need for a toxic contrast agent injection.

Shir Filo, explained, “Our technology opens up new possibilities for understanding the role of iron in cancer, normal aging, and neurodegenerative diseases. It enables non-invasive research and diagnosis of iron homeostasis in the living human brain, offering a potential game-changer for healthcare and neuroscience.”

New MRI Technique. Credit Shir Filo Team
New MRI Technique. Credit Shir Filo Team

Prof. Aviv Mezer emphasized, “The strength of our team lies in its diverse composition, comprising experts from various disciplines and specialties. Dr. Tal Shahar was leading the medical aspect of the study involving meningioma patients and proteomic studies. Dr. Rona Shahrabani was instrumental in conducting experiments with iron proteins, while Dr. Nevo Margalit, Chair of the Neurosurgery Department at Shaare Zedek Medical Center, made substantial contributions through his involvement in a significant portion of the surgeries and recruitment of patients. Additionally, Dr. Eli Ben David, a radiologist at Shaare Zedek Medical Center, played an indispensable role in enabling the MRI scans and Dr. Naomi Habib brought valuable knowledge in gene expression. Their collective and truly interdisciplinary expertise and unwavering dedication significantly enhanced the success of this pioneering study.”

The study, titled “Non-invasive assessment of normal and impaired iron homeostasis in the brain,” was published in Nature Communications. The DOI for the paper is 10.1038/s41467-023-40999-z.

Date of publication: September 12th 10:00 London Time – 05:00 ET

Research Team: Shir Filo-1, Rona Shaharabani-1, Daniel Bar Hanin-1, Miriam Adam-1, Eliel Ben-David-2, Hanan Schoffman-3, Nevo Margalit-4, Naomi Habib-1, Tal Shahar3,4,5, Aviv A Mezer-1

1-The Edmond and Lily Safra Center for Brain Sciences, The Hebrew University of Jerusalem, Israel

2-The Department of Radiology, Shaare Zedek Medical Center, Faculty of Medicine, The Hebrew University of Jerusalem, Israel

3-The Laboratory of Molecular Neuro-Oncology, Shaare Zedek Medical Center, Faculty of Medicine, The Hebrew University of Jerusalem, Israel

4-The Department of Neurosurgery, Shaare Zedek Medical Center, Faculty of Medicine, The Hebrew University of Jerusalem, Israel

5-The Department of Neurosurgery, Tel Aviv Sourasky Medical Center, Affiliated with Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel