August 23, 2022 – Despite advances in increased food production, half of all world’s harvested food is lost due to rot caused by microorganisms. Plants emit various volatile organic compounds into their surrounding environment, which can be monitored for early detection of plant disease and to prevent food loss.
A new research study led by the Hebrew University of Jerusalem (HU) and Israel’s Agricultural Research Organization (Volcani Institute) details the success of a biological sensor for the early detection of hidden disease in potato tubers, one of Israel’s chief export industries at 700,000 tons a year.
Israeli farmers import European potatoes for planting in Israel. However, a certain percentage of them carry diseases within—either visibly or invisibly—that cause rot and significantly reduce the potato’s quality. The Hebrew University-Volcani alliance is about to change that. They developed a sensor that detects disease and can be used to inhibit the rot from growing and spreading. Their study, published in the upcoming edition of Talanta, was conducted by Dr. Dorin Harpaz and her Ph.D. student Boris Veltman at HU’s Robert H. Smith Faculty of Agriculture, Food, and Environment, under the supervision of Dr. Evgeni Eltzov of the Volcani Institute. The team collaborated with the Volcani Institute’s Dr. Sarit Melamed and Dr. Zipora Tietel, as well as Dr. Leah Tsror from the Gilat Research Center.
The sensor relies on smart bioengineering and optics. When the sensor is exposed to an infected potato, a bacterial compound within lights up—with the strength of the luminescence indicating the concentration and composition of the rot. “The intensity of the light given off by the bacteria panel makes it possible to quickly and quantifiably analyze the characteristics of the disease, which the sensor can ‘smell,’ before the appearance of visible symptoms,” explained Eltzov. “The biosensor we developed will help identify diseased potatoes that do not yet have any external indications, and keep them away from healthy tubers, thus preventing the rot from developing or spreading to other healthy plants,” Harpaz added.
To form the bacteria panel, the team created a compound of four genetically-engineered bacteria that measure biological toxicity. In this study, the biological sensor detected disease before there was any visible trace, and caused the optical sensor to shine twice as brightly as did the sensors in non-infected potatoes. Their capabilities were also demonstrated in a previous study that used the sensors to detect toxicity among artificial sweeteners in sports supplements.
According to the researchers, early discovery of disease—before the potatoes are exported to foreign markets or replanted, offers a significant advantage to food growers. “The biological sensor can be used to quickly and economically identify hidden rot in potatoes, facilitate better post-harvest management, and reduce food wastage—particularly important given the current global food crisis,” concluded Harpaz.
CITATION: Boris Veltman, Dorin Harpaz, Sarit Melamed, Zipora Tietel, Leah Tsror, Evgeni Eltzov, Whole-cell bacterial biosensor for volatile detection from Pectobacterium-infected potatoes enables early identification of potato tuber soft rot disease, Talanta, Volume 247, 2022, 123545 DOI 10.1016/ j.talanta.2022.123545.
FUNDING: ICA Charitable Association, Chief Scientist of the Agricultural Ministry, Israel.